Produkt: CCV (Canine Corona Virus) IgG Ab
CCV (Canine Corona Virus) IgG Ab
: serum, plasma
: 3-step dilution
: 100µl of diluted samples
: 1h (37°C), 1h (37°C), 15min at RT
: optical density at 450nm
For Veterinary Use Only!
Canine Corona Virus (CCV) is an important and complex disease of both wild and domestic dogs. The great majority of dogs that become infected recover completely and develop immunity to CCV. Some of the recovered dogs become carriers of the virus and can infect other dogs. A few infected dogs do not build up immunity to CCV and the disease progress to a fatal form. The fatal, disseminated form of CCV is a chronic, progressive disease characterized by diarrhea, intestinal disease, weakness, loss of appetite.
Important in the diagnosis of CCV are: - clinical history
- clinical signs
- laboratory findings: - antigen detection
- antibody detection
This test measures coronavirus antibodies which are present in the blood or plasma. Most antibody positive dogs (especially those with intermediar titers) are possible virus carriers and may shed CCV.
Intended use of the test kit
The CCV ELISA test kit is designed to detect antibodies against CCV proteins (mostly glycoproteins). CCV proteins are attached to the solid phase. After washing the strips are incubated with the dog sera to be tested. The strips are washed after incubation to remove unbound materials. A HRPO labeled anti-species conjugate is added to detect bound dog antibodies to CCV proteins. After incubation and rinsing the substrate is added and the optical density is measured at 450 nm.
Principle of the test kit
The test is based on the reaction of CCV proteins (mostly glycoproteins) with polyclonal dog antibodies. To this end CCV proteins have been coated to a 96-well microtiter plate.
The diluted dog serum/plasma sample is added to the wells of the coated plate.
After washing the bound dog antibodies are detected by a HRPO conjugated antispecies conjugate.
The color reaction in the wells is directly related to the concentration of CCV antibodies in the serum/plasma sample.