Produkt: Respiratory syncytial virus (RSV) IgM ELISA
Respiratory syncytial virus (RSV) IgM ELISA
Serum or Plasma
1 – 150 U/ml
60 min, 30 min, 20 min at RT
TMB at 450 nm
Special remarks: The DEMEDITEC RSV IgM Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgM antibodies against Respiratory syncytial virus in serum and plasma.
The antigen used for coating the plates is Strain Long, F-protein.
Each year during the winter months, RS viruses spread heavily amongst children and infants. There are recurring infection by RS viruses every year. Voluntary studies with adults have demonstrated that a reinfection with RS viruses is possible. The incubation time is 2 - 6 days. RS viruses are paramyxoviruses with a diameter of 90 - 140 nm. The most noticeable connection of RSV infections with respiratory infections and specific clinical syndromes was detected in infants up to 6 months of age with bronchiolitis or pneumonia. In older infants or small children the disease is milder. In 25 % of infections of the respiratory tract RSV infections are detectable. As reinfections with RSV are possible, it is assumed that these reinfectious antibodies are responsible for the mild course of the disease in adults, being similar to a cold. However, especially in the early years, serum antibodies are no effective protection against infections of the respiratory tract. Therefore, this pathogen may cause bronchiolitis or, in infants up to 4 months of age, pneumonias. Based on their antigen relationship, RSV isolates are differenciated into two major groups (A and B). The surface glycoproteins of the virus (G glycoprotein and fusion glycoprotein) cause the production of virus-neutralizing antibodies. Obviously the G glycoproteins of groups A and B are very different, while the F glycoproteins of both groups show a high antigen concurrence. The complement binding reaction is unsatisfactory for the serological diagnosis of RSV. Enzyme immunoassays are of diagnostic value for the serological diagnosis of RSV infections, as they are very sensitive and allow the differentiation of antigens into the various immunoglobulin classes. In RSV infections, it is possible that the IgM antibody response is missing or so weak that a reliable interpretation of the results is impossible. The detection of IgG antibodies in a single sample is no evidence for an acute infection as, in some patient IgA, antibodies may persist months and years. The method recommended for serological testing of acute RSV infections is the determination of IgG antibodies in serum pairs with significant titer rise.
In order to avoid interference of rheumatoid factors, it is highly recommended that patient sera should be treated with RF absorbent (Cat.No. DE-MJS02). Alternatively, positive results can be confirmed in a second test run in the presence of RF adsorbent. Do not pre-treat the controls or standards with RF absorbent!