Special remarks:

The DEMEDITEC Adenovirus IgM Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgM antibodies against Adenovirus in serum and plasma.
The antigen used for coating the microtiterplates is Adenovirus hexon of type 5, strain adenoid 75.

The adenovirus is an ubiquitous pathogen of humans and animals. Adenoviruses are characterized by location inside the cell nucleus, common complement-fixing antigens and marked stability to environmental effects. Adenoviruses are endemic in all populations throughout the year. The infection is spread both through the aerial-droplet route and the routes characteristic for intestinal infections. The incubation period is between five and seven days. Adenoviruses mainly infest respiratory and intestinal mucosa, but also the cornea. They are accumulated in the epithelial cells and regional lymph nodes. Adenoviruses cause the widest variety of illnesses of the known respiratory viruses. The adenovirus infection is the most frequently caused viral disease of the respiratory tract among preschool children (types 1 - 5 and 7). Acute diseases of the upper respiratory tract occur predominantly. Pneumonia is the most severe form of adenoviral infection occurring mostly in infants below the age of one. Adenoviruses also cause outbreaks of swimming-pool-associated pharyngoconjunctival fever in the summer and epidemics of kerato-conjunctivitis of both children and adults. The intestinal form of adenoviral infection occurs mostly in children below the age of one. An acute adenoviral infection can be detected by virus isolation and/or serology. The serologic tests are particularly important because they document actual infection in the patient and can be applied to large-scale epidemiologic investigations. The CF and ELISA tests measure predominantly the antibodies directed against the group-specific determinants on the hexon component. The recommended tests for measuring type-specific antibodies are hemagglutinin inhibition and serum neutralization. The type-specific antigenic determinants of adenoviruses are located at the fibers on the capsid. Because of the ubiquity of the adenoviruses and numerous cross-reactions between related serotypes, seroconversion involving a fourfold or greater rise in antibody infection is necessary to document infection. IgG is the predominant antibody class measured in the serologic tests.

In order to avoid interference of rheumatoid factors, it is highly recommended that patient sera should be treated with RF absorbent (Cat.No. DE-MJS02). Alternatively, positive results can be confirmed in a second test run in the presence of RF adsorbent. Do not pre-treat the controls or standards with RF absorbent!