Special remarks:

INTRODUCTION
Angiopoietin-like proteins (ANGPTLs) are secretory form proteins which are similar in structure to angiopoietin which is an angiogenesis factor, and 7 ANGPTLs have been identified. ANGPTL2 has been found to affect vascular cells and monocytes, and it have also been known that ANGPTL3 and ANGPTL4 play important roles in llipid  metabolism  and  AGF  (Angiopoietin-like  growth  factor)/ANGPTL6  plays  in energy  metabolism.  These  various  biological  effects  of  ANGPTL  family  are attracting attention as a new target of strategy against lifestyle-related diseases like metabolic  syndrome  or  cancer.  There  is  a  report  that  high  level  of  ANGPTL2  in blood observed in individuals who suffer adipsity, strong insulin resistance, diabetes and arteriosclerosis.  
This kit is a quantitative ELISA kit which can measure ANGPTL2 concentration in human serum or plasma.
 
PRINCIPLE 
This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies.
Tetra  Methyl  Benzidine  (TMB)  is  used  as  a  coloring  agent  (Chromogen).  The strength of coloring is proportional to the quantities of human ANGPTL2.
 
INTENDED USE
For research use only, not for use in diagnostic procedures. 
■  This product is capable for the quantitative determination human ANGPTL2 in serum, EDTA-plasma and cell culture supernatant.
■  The recommend dilution for human serum and EDTA-plasma samples is about 10-fold with “4, EIA buffer”.