Special remarks:

Intended use and principle of the test
Enzyme Immunoassay for the quantitative determination of Metanephrine and Normetanephrine in urine.

First Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are quantitatively acylated.

The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is
monitored at 450 nm.

Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.
During the sample preparation Metanephrine (Metadrenaline) is quantitatively acylated.