Special remarks:

Intended use and principle of the test
Enzyme Immunoassay for the quantitative determination of Chromogranin A in serum and plasma.

The quantitative determination of Chromogranin A (CgA) follows the basic principles of the enzyme immunoassay.

First, the Chromogranin A in the samples, controls and standards binds to CgA-specific antibodies fixed to a 96 wells microtiter plate. A sandwich is formed by adding biotinylated CgA antibodies. The wells are washed thoroughly and incubated with horseradish peroxidase conjugated streptavidin. After another washing step the complex bound to the solid phase is detected by using TMB as a substrate.

The reaction is monitored at 450 nm.
By means of a calibration curve the CgA concentrations in the samples are determined.