Leptin mouse/rat ELISA

DEBV1007


Enzyme immunoassay for the quantitative measurement of mouse and/or rat leptin in serum and plasma

MethodELISA
Tests96
Incubation Time / Conditions1 h, 1h, 30 min (RT/shaker), 10 min (RT)
Standard Range100 - 4,000 pg/ml
Sensitivity analyticalLoD: 30 pg/ml (mouse); 50 pg/ml (rat)
Final Sample Volume100 µl
Sample Typeserum, plasma (EDTA, citrate, heparin)
Sample Pretreatmentpre-dilution
Isotope / SubstrateTMB 450 nm
kBq-
Internal Controls2
Regulatory StatusRUO